P9103-40E-HRP

Protein Kinase C, zeta, Mid (PKCz) (HRP)

Size

£914.00

SKU:: P9103-40E-HRP
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide derived from the middle region of the catalytic domain of the mouse protein kinase C, zeta isoform (PKCz) protein.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Extra Details:: PKC isoforms are divided into three classes: conventional (cPKC), novel and atypical (aPKC).1 Vertebrates express two atypical PKCs, z and i/l. PKCi2 and -l3 are human and mouse orthologues, respectively. PKCz ia a key regulator of signaling pathways, and is involved in the MAP kinase cascade, NFkB activation, ribosomal S6-protein kinase signaling, and cell polarity.Brain-specific PKCz, or PKMz, is a new PKC isoform, synthesized from PKMz mRNA encoding a PKCz catalytic domain without a regulatory domain, which is not a proteolytic fragment of PKCz, as previously thought. PKMz is a specific, autonomously active form of aPKCz4 that is both necessary and sufficient for maintaining hippocampal long-term potentiation (LTP). PKCz is the only PKC isozyme with a stable PKM form in hippocampus.6,7 Metabolic labeling of hippocampal slices shows increased de novo synthesis of PKMz in LTP. Because PKMz is a kinase synthesized in an autonomously active form and is necessary and sufficient for maintaining LTP, it serves as an example of a link coupling gene expression directly to synaptic plasticity. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunoprecipitation: 7ug/IP reaction Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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