P9103-25D

Protein Kinase C, gamma (PKCg)

Size

£709.00

SKU:: P9103-25D
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified rat brain PKC.
Clone:: 3G257
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Protein Kinase C (PKC), one of the most important proteins in signal transduction, is involved with cell proliferation, differentiation and function. PKC is a family of Ca++/phosphatidylserine-dependent, serine/threonine kinases with 12 known isozyme members (a, bI, bII, g, d, e, z, h, q, l, m, i). PKC isozyme expression and distribution varies at both the tissue and intracellular levels. PKC is activated by diacylglycerol. PKC functions are dependent on isozyme type and location. PKC g (gamma, PKC type I) has a MW of 78kD. It is found predominantly in the brain and spinal cord. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:2000 Immunohistochemistry (Paraffin): 1:25-1:100 Optimal dilutions to be determined by the researcher. Source: Ascites Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.