P9103-16T

Protein Kinase C alpha, phosphorylated (Ser657/Tyr658) (PRKCA)

Size

£576.00

SKU:: P9103-16T
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH conjugated peptide corresponding to amino acid region encompassing the human phospho-PKC alpha (Ser657/Tyr658). Sequence highly conserved in mouse and rat.
Format:: Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Extra Details:: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The Nterminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (a, bI, bII, and g) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligandstimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCbI and PKCa, respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases. Western blot analysis of immunoprecipitates from neonatal rat brain lysate using an anti-PKCa antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCa and anti-phospho-PKCa (Ser-657/Tyr-658). The latter shows no detection of PKCa after phosphatase treatment. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice