P9103-16B2

Protein Kinase C (PKC)

Size

£633.00

SKU:: P9103-16B2
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified bovine brain PKC.
Clone:: 3C174
Format:: Supplied as a liquid in Tris-Glycine, 0.15M sodium chloride, 0.05% sodium azide
Extra Details:: Protein Kinases C (PKC) are a family of serine/threonine protein kinases that play key roles in extracellular signal transduction. MC5 blocks partial proteolysis of PKC by trypsin in vitro. Inappropriate activation of PKC has been implicated in the pathophysiology of many diseases, including diabetes mellitus. The PKC family now includes 11 isoforms that are differentially expressed in various cell types. Applications: Suitable for use in RIA, Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:1000. Immunohistochemistry (Frozen/paraffin): 1:200-1:500. Immunofluorescence (IC): 1:100-1:500. ELISA: 1:100-1:2000. Western Blot: 1:1000. Immunoprecipitation: 1:10-1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.