P9102-91A
Protein Kinase A, NT (PKA)
Size
£761.00
- SKU:
- P9102-91A
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- 16 residue synthetic peptide (KKGSEQESVKEFLAK-C) based upon amino acids 8-22 of the human 41kD alpha isoform of the catalytic subunit of PKA. The immunizing sequence is identical in mouse, rat, hamster, bovine and porcine.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Extra Details:
- The action of many hormones is mediated by the generation of the intracellular second messenger cAMP. The predominant effect of cAMP is to activate PKA (cAMP-dependent protein kinase). Four molecules of cAMP bind each dormant PKA holoenzyme activating the kinase by releasing the catalytic (C) subunits from the regulatory (R) subunit-cAMP complex. The cellular location of PKA is dictated by the R subunit. Two classes of R subunit exist; RI and RII, which form the type I and type II PKA holoenzymes respectively. The RI isoforms (RIa and RIb) are thought to be primarily cytoplasmic whereas a significant proportion of the RII isoforms (RIIa and RIIb) are particular and associate with the plasma membrane, cytoskeletal components, endoplasmic reticulum, secretory granules or nuclei. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detected PKA, NT (42kD) in both rat brain microsomal preparation and PC-12 RIPA cell lysates. Rat brain preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with P9102-91A (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice