P9102-70J
Protein Gene Product 9.5 (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin thiolesterase, PARK5)
Size
£581.00
- SKU:
- P9102-70J
- Application:
- WB
- Purity:
- Purified by peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Asp110 of the human Protein Gene Product 9.5.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Protein ubiquitination and deubiquitination is a reversible process catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs). DUBs are categorized into 5 subfamilies USP, UCH, OTU, MJD and JAMM. UCHL1 and UCHL3 belong to the UCH family of DUBs. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 is more widely distributed. UCHL1 binds monoubiquitin and UCHL3 shows affinity to both ubiquitin and NEDD8, a ubiquitin-like molecule. Both enzymes play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice