P9102-70H
Protein Gene Product 9.5, Recombinant, Human (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin thiolesterase, PARK5)
Size
£512.00
- SKU:
- P9102-70H
- Molecular Weight:
- 24.8
- Purity:
- ≥95% by SDS PAGE
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris-HCl pH8.0, 2mM EDTA.
- Extra Details:
- PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques. Molecular Weight: ~24.8kD (223 a.a) Amino Acid Sequence: MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA Enzyme Activity: > 150 pmol/min/ug, Unit Definition: efined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice