P9102-29A
Protein C
Size
£696.00
- SKU:
- P9102-29A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Protein C isolated from human plasma.
- Clone:
- 6F183
- Format:
- Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
- Extra Details:
- Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kD proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55kD and the normal concentrations in human plasma is approximately 1-3ng/ml because of the very fast turnover, the proenzyme concentration is approximately 3ug/ml. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Strong reaction is seen in capture ELISA in combination with a biotin labeled antigen against Protein C only when using a Tris-buffer containing 2.5mM CaCl2 Western Blot: After SDS-PAGE, reacts with protein C in non-reduced form only. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice