Protein G (Not for Export EU)

Size

£626.00

SKU:: P9101-05
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Protein G [Streptococcus species]
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 2ml sterile ddH20
Extra Details:: Protein G is a small globular cell surface protein produced by Streptococcus sp. It is composed of two or three nearly identical domains of 55 amino acids each. It binds to Fc fragment of IgG with high affinity. Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Sandwich ELISA: 1:20,000-1:100,000. Assayed against 1ug of P9101-05 using Pab Gt xRb IgG, H&L (HRP) (I1904-46Q) and ABST (A0030) as a substrate for 30 minutes at RT. Western Blot: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.