P9075
Protease, S. aureus V8 (Endoproteinase Glu-C)
Size
£358.00
- SKU:
- P9075
- Molecular Weight:
- 27
- Purity:
- Chromatographically purified
- Storage Conditions:
- 4°C
- Supplier:
- United States Biological
- CAS Number:
- 66676-43-5
- Format:
- Lyophilized powder
- Extra Details:
- Endoproteinase-Glu-C Protease from S. aureus V8 specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acid. In the presence of ammonium buffers, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. It is is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3-. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976). Activity: ≥500 units per mg dry weight Absorbance (A280) at 1mg/ml: As Reported Unit Definition: One Unit causes a change of 0.001 absorbance unit (at 280nm) per minute at 37°C, pH 7.8 using casein as the substrate. Extinction Coefficient: 4.26 Optimum pH: 4.0 and 7.8 with hemoglobin substrate Inhibitors: Diisopropyl fluorophosphate (DFP) and monovalent anions such as F-, Cl-, Br-, CH3COO-, and NO3- Quality Control: SDS-PAGE Storage and Stability: Stable for 6 months after receipt at 4ºC. Autolysis occurs at temperatures greater than 40ºC. The enzyme is fully active in 2mg/ml SDS.
- Shipping Conditions:
- Blue Ice