P9054-75C

Prostatic Acid Phosphatase, Recombinant, Human, His-Tag (ACPP, 5'-nucleotidase, 5'-NT, TMPase, Ecto-5'-nucleotidase, Thiamine Monophosphatase)

Size

£832.00

SKU:: P9054-75C
Molecular Weight:: 41
Purity:: ≥95% by SDS-PAGE under reducing conditions and visualized by colloidial Coomassie Blue stain at 5ug per lane. Endotoxin: ≤1EU/ug (LAL)
Storage Conditions:: 4°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris, 150mM sodium chloride, pH 7.5
Extra Details:: Prostatic Acid Phosphatase (ACPP) catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins. The activity optimum of ACPP is in the pH range of 4-6, and the activity is inhibited by L(+)-tartrate. ACPP expression levels are highest in the prostate, with much lower expression in most other tissues. ACPP is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Cellular ACPP has been shown to be a protein tyrosine phosphatase. Protein substrates include the epidermal growth factor receptor and HER-2. Cellular ACPP is considered to function as a tumor suppressor. Recombinant corresponding to Met1-Gln379 from human ACPP, fused to 6-His-tag at C-terminal expressed in mouse myeloma cell line, NS0. Biological Activity: Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). Specific Activity: >100,000pmol/min/ug Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice