Prostate Specific Membrane Antigen (PSMA, PSM, Folate Hydrolase, FOLH, FGCP, Glutamate Carboxypeptidase 2, GCP2, Membrane Glutamate Carboxypeptidase, mGCP, N-acetylated alpha-linked Acidic Dipeptidase 1, NAALAD1, NAALADase I, NAALAdase, Pteroylpoly gamma Glutamate Carboxypeptidase)

Size

£889.00

SKU:: P9054-63M
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Lys44-Ala750 of human Prostate Specific Membrane Antigen expressed in CHO cells.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Human prostate specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma-lutamates, facilitating the uptake of folate (4). Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml using human prostate tissue under reducing conditions. Flow Cytometry: 2.5ug/10e6 cells using LnCAP human prostate cancer cell line Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human prostate cancer tissue Optimal dilutions to be determined by the researcher. Matched Pair: Detection: P9054-63M Capture: 059356 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice