P9054-08B
Prostate Specific Antigen (PSA, Kallikrein-3, Kallikrein III, Seminin, Semenogelase, γ-Seminoprotein, P-30 antigen, KLK3)
Size
£728.00
- SKU:
- P9054-08B
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human Prostate Specific Antigen
- Clone:
- 3H262
- Format:
- Supplied as a lyophillized powder in 0.01M PBS, pH 7.0. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. Anti-PSA is useful in the positive identification of metastatic deposits of prostatic origin. Applications: Suitable for use in ELISA, Western blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA (Capture Antibody): 0.5-1ug/well. Detects ~1ng/well in the range of 0-200ng/ml. Recommend using TMB substrate. Western Blot:10ug/ml. Detects 1ug/lane of human PSA. Immunohistochemistry: Formalin-fixed paraffin embedded and frozen tissue sections using ABC immunoperoxidase assay. Optimal dilutions to be determined by researcher. Matched Pair: Suitable for use with P9054-08A in a sandwich ELISA. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice