Properdin (Complement Factor P, CFP, PFC) (Not for Export EU)

Size

£466.00

SKU:: P9051-10E
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Properdin isolated from pooled human plasma
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Extra Details:: Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20mg/ml. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Single or Double Radial Immunodiffusion. Other applications not tested. Recommended Dilution: Immunoelectrophoresis: 120ul against 2ul human serum. Single Radial Immunodiffusion: 1% in the gel. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 10ul in 3mm diameter peripheral wells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.