Prohibitin 2

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£876.00

SKU:: P9007-53
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: E. coli-derived rhPHB2. (aa 89 - 299)
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Prohibitin 2 (PHB2; also B-cell receptor-associated protein BAP37 and REA) is a 37kD, ubiquitous member of the prohibitin family of molecules. PHB2 is found in multiple intracellular compartments. It is suggested to be a component of an inner mitochondrial membrane complex and a nuclear repressor of the estrogen receptor (ER). Human PHB2 is 299 amino acids (aa) in length. It contains a mitochondrial localization signal (aa 1-50), two ER repressor domains (aa 19-49 and aa 150-174), and a central coiled-coil domain (aa 190-238). There may be one splice variant that shows a premature truncation after aa 289. Over aa 89-299, mouse and rat PHB2 are absolutely (100%) identical to human PHB2. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhPHB2 is ~ 6.5ng/well. Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice