P9007-51
Prohibitin
Size
£1,010.00
- SKU:
- P9007-51
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant protein corresponding to rat prohibitin. Cellular Localization: Cytoplasmic.
- Clone:
- 0.N.532
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- Prohibitin is an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. PROHIBITIN APPEARS TO BE A VERY RELIABLE MARKER OF MITOCHONDRIA. Shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 2ug/mg protein lysate, (Protein G). Native and denatured Western Blot: 0.5-1ug/ml for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: MAD109, or PC12 cells, heart Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice