Progesterone Receptor B, phosphorylated (S294) (NR3C3, PR-B)

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£735.00

SKU:: P9007-31J
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Phosphopeptide containing human PR-B S294 site
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Progesterone Receptor B (PR-B) is a 118kD member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR-B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region aa 1-566), a DNA binding domain (aa 567-639), and a steroid-binding region aa 681-933). Ligand binding induces a key phosphorylation event at Ser294 catalyzed by ERK1/2, regulating cell growth, cell adhesion, and gene transcription. An alternate start site at Met165 generates 90kD PR-A, an isoform particularly important in the ovary and uterus that insures fertility. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml with lysates of T47D human breast cancer cell line untreated (-) or treated (+) with 100 nM synthetic progestin promegestone for 60 minutes under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice