P9007-15H
Progesterone Receptor, phosphorylated (Ser190)
Size
£696.00
- SKU:
- P9007-15H
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser190 of human progesterone receptor (KLH)
- Clone:
- 1154
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, 50% glycerol.
- Extra Details:
- Progesterone Receptor (PR) is phosphorylated on multiple serine residues located in the N-terminal domain and in the hinge region between the DNA and steroid binding domains. The activity of PR can be regulated by its phosphorylation by cyclin-A/Cdk2, casein kinase II, and MAP kinase. Individual sites such as Ser190 enhance transcriptional activity, while Ser294 seem to be a site of convergence and regulation by crosstalk with other signaling pathways. Of the seven identified serine residues on which the human PR (hPR) is phosphorylated, four (Ser81, Ser162, Ser190 and Ser400) are basally phosphorylated and exhibit a rapid increase in phosphorylation in response to treatment with hormone. The other three sites (Ser102, Ser294 and Ser345) are hormone-inducible and reach maximal phosphorylation within 1-2 hours of treatment. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:1000 Western Blot: 1:1000 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice