Profilin, Inflammatory, T. gondii (Profilin-like)

Size

£863.00

SKU:: P9005-84
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Ser2-Tyr163 from T. gondii Profilin expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: T. gondii Profilin is a 163 amino acid, 18kD profilin-like protein that generates a potent interleukin-12 (IL-12) response in murine dendritic cells through binding to Toll-like receptor 11 (TLR11). TLR11 does not appear to be expressed in human. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml with the appropriate secondary reagents detects T. gondii Profilin. The detection limit for T. gondii Profilin is ~1ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.