P9005-45
Procathepsin K
Size
£607.00
- SKU:
- P9005-45
- Purity:
- Purified by Protein G affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to 314aa with a 12aa His-tag from human Procathepsin K expressed in E. coli.
- Clone:
- 5i345
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. Clarify the solution by centrifugation.
- Extra Details:
- Human cathepsin K is a member of the cysteine protease family. It has been shown that cathepsin K plays a major role in the resorption of the bone matrix by osteoclasts. Cathepsin K has potential as a drug target for the diseases related to bone matrix metabolism. Autoproteolytic processing of the N-terminal 99 aa propeptide produces the active, mature form of cathepsin K. It is presumed that the activation of procathepsin K in vivo occurs in the bone resorption pit, which has a low-pH environment Humans lacking cathepsin K exhibit pycnodysostosis, which is characterized by short stature and osteosclerosis. Factors that directly modulate osteoclastic bone resorption, including cytokines (RANK ligand, tumor necrosis factor-alpha and interferon gamma), hormones (retinoic acid and estrogen) and nuclear transcriptional factors (c-jun and Mitf) also regulate cathepsin K gene expression. Cathepsin K is an attractive target for therapeutic intervention to prevent and ameliorate the significant deleterious impact of osteoporosis. In the cartilage, cathepsin K has a potent aggrecan-degrading activity and cathepsin Kgenerated aggrecan cleavage products specifically potentiate the collagenolytic activity of cathepsin K toward type I and II collagens. The osteoclast is not the only cell type expressing cathepsin K: It was found in epithelloid cells and multinucleated giant cells irrespective of the pathological condition and anatomical location, but not in normal resident macrophages. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice