P9004-26A
PRMT5 (SKB1 homolog, HMT1 hnRNP methyltransferase-like 5, SKB1Hs, SKB1, JBP1, IBP72, HRMT1L5, protein arginine methyltransferase 5)
Size
£642.00
- SKU:
- P9004-26A
- Application:
- WB
- Purity:
- Purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to RACREKDRDPEAQ, from the internal region of the protein sequence according to (NP_006100.2; NP_001034708.1.).
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of mega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice