P9003-69

PRL-2, aa2-167, catalytic domain Recombinant, Human (Protein tyrosine phosphatase, Phosphatase of regenerating liver-2)

Size

£951.00

SKU:: P9003-69
Purity:: ≥95% by SDS-PAGE.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25 mM Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 2mM EDTA, 1 mM DTT, 10 mM glutathione.
Extra Details:: Human PRL 2 (GenBank Accession No. NM_080391), a.a. 2-167 with N-terminal His-tag, MW = 19.1 kDa, expressed in an E. coli expression system. Specific Activity: 1 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30°C Assay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.