P8950-10
Prion Protein, aa109-112 (PrP, CD230)
Size
£889.00
- SKU:
- P8950-10
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa109-112 of human prion protein.
- Clone:
- 2Q126 (3F4)
- Format:
- Supplied as a liquid in PBS. No preservatives added.
- Extra Details:
- The prion protein is a large membrane protein that occurs normally in neurons of the human brain and is thought to be involved in synaptic transmission. In prion diseases, such as CJD, Gerstmann-Straussler-Scheinker syndrome (GSS), Fatal Familial Insomnia (FFI), Alpers Syndrome and Kuru, the normal cellular form of this protein (PrPc) is transformed into an altered protein when it comes into contact with an infectious prion protein (PrPsc) from another host. This altered PrPsc accumulates in cytoplasmic vesicles of diseased individuals forming lesions, vacuoles and amyloid deposits. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:100-1:1000 Requires citrate buffer (HIER). Immunoreactivity is seen predominantly as cell body staining of neurons. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures: a. formic acid for 10 minutes at RT b. hydrolytic autoclaving c. microwaving Western Blot: 1:10,000-1:100,000 Immunoprecipitation: 1:10-1:100 ELISA: ≥1:100,000 Optimal dilutions to be determined by the researcher. Recommended Control: Brain tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice