P6300-35C
Presenilin 1, NT
Size
£767.00
- SKU:
- P6300-35C
- Purity:
- Supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Fusion protein corresponding to residues 21-80 at the N-Terminal of human Presenilin 1 with a GST-Tag.
- Clone:
- 2Q127
- Format:
- Supplied as a liquid. No preservatives added.
- Extra Details:
- Immunohistochemical studies of control brains revealed that PS1 is expressed primarily in neurons, with the protein localizing in the soma and dendritic processes. In contrast in FAD and sporadic Alzheimer’s disease cases, PS1 immunoreactivity was present in the neuritic component of senile plaques as well as in neurofibrillary tangles. Localization of PS1 immunoreactivity in familial and sporadic Alzheimer’s disease suggest that genetically heterogeneous forms of the disease share a common pathophysiology involving PS1 protein. By Western blot recognizes a predominant 32kD polypeptide in a variety of samples, including PC12 cells transfected with human PS1 complementary DNA, brain biopsy specimens from demented patients and postmortem samples of frontal neucortex from Familial Alzheimer’s Disease cases, late-onset Alzheimer’s disease cases and cases of other degenerative disorders. Applications: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:250-1:500. Recommend blocking buffer is TBS containing 5% non-fat milk and 0.01% Tween-20. It is recommended that you also dilute the antibody in this blocking buffer. Incubate for 1 to 2 hours at RT or overnight at 4°C. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice