P6000-87
Pregnancy Associated Plasma Protein A (PAPP-A, Pappalysin 1, PAPA, PAPPA, PAPP-A, PAPPA1, Aspecific BCL2 ARE-binding Protein 2, ASBABP2, Differentially Placenta 1 Expressed Protein, DIPLA1, EC 3.4.24.79, IGF-dependent IGFBP-4 Protease, IGFBP-4ase, Insulin-like Growth Factor-dependent IGF-binding Protein 4 Protease, Pappalysin 1 Precursor)
Size
£895.00
- SKU:
- P6000-87
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human PAPP-A/proMBP complex
- Clone:
- 5E741
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- PAPP-A was initially identified as a high molecular mass component in human pregnancy serum. In pregnancy serum PAPP-A exists in a disulfide bound heterotetrameric complex consisting of two 200kD PAPP-A subunits and two 50-90kD subunits of the proform of eosinophil major basic protein (proMBP). The concentration of PAPP-A in serum increases with gestational age, however, in Down’s syndrome pregnancies PAPP-A concentration is markedly reduced in the first trimester. As such, PAPP-A is widely used as an early marker for Down’s syndrome. PAPP-A has also been shown to exist in a homodimeric, 400kD form in unstable atherosclerotic plaques. Increased circulating concentrations of this dimeric PAPP-A are associated with acute coronary syndromes. Applications: Detection of human PAPP-A antigen in ELISA, Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Matched Pair for the detection of PAPP-A in blood of AMI patients and patients with unstable angina (Capture-Detection): P6000-84 – P6000-86, P6000-84 – P6000-87, P6000-82A – P6000-80A Matched Pair for the detection of PAPP-A in pregnancy serum (Capture – Detection): P6000-84 – P6000-86, P6000-84 – P6000-87, P6000-85 – P6000-86, P6000-82A – P6000-80A, P6000-82A – P6000-81A, P6000-81A – P6000-82A Hybridoma: Derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP complex. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice