P5750-58A
PRDM14 (PR Domain Zinc Finger Protein 14, PR Domain-containing Protein 14)
Size
£780.00
- SKU:
- P5750-58A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to PRDM14 expressed in E. coli (Q9GZV8).
- Clone:
- 11D13
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- PRDM14 is an approximately 65kD transcriptional inhibitor that contains one histone methylating PR/SET domain (aa266-367) and six zinc finger repeats(aa400-568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up-regulated in breast cancer and lymphoblastic leukemia. Within (aa1- 201), human and mouse PRDM14 share 46% aa sequence identity. Applications: Suitable for use in Direct ELISA, Western Blot.. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice