POU2F3 (POU Domain, Class 2, Transcription Factor 3, Octamer-binding Protein 11, Oct-11, Octamer-binding Transcription Factor 11, OTF-11, Transcription Factor PLA-1, Transcription Factor Skn-1, OTF11, PLA1)

Size

£642.00

SKU:: P5250-06A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-EPHLEASQHLPVPKH, from human POU2F3, at the internal region of the protein (NP_055167.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 1-3ug/ml, lysates of cell lines HEK293 and HepG2 observed on ~50kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.