POU2F1 (POU Domain, Class 2, Transcription Factor 1, NF-A1, Octamer-binding Protein 1, Oct-1, Octamer-binding Transcription Factor 1, OTF-1, OCT1, OTF1)

Size

£642.00

SKU:: P5250-01K
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-NVQSKSNEESGDSQQ, from human POU2F1, at the internal region, near N-terminal (NP_002688.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: OCT1 is a transcription factor that binds the octamer motif 5'-ATTTGCAT-3' in promoters of various genes.OCT1 contains one homeobox DNA binding domain and is part of the POU transcription factor family due to the presence of one POU domain, a highly charged motif that selectively binds the octamer motif.OCT1 is also known as POU domain class 2 transcription factor, octamer-binding transcription factor, oct-1, OTF-1, NF-A1, and POU2F1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:4000 Western Blot: 0.5-2ug/ml, nuclear lysates of cell line HeLa observed on ~100kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.