P4700-13-ML490
PSD95, Postsynaptic Density Protein 95 (PSD-95, PSD95, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90) (MaxLight 490)
Size
£1,151.00
- SKU:
- P4700-13-ML490
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human PSD95. Species sequence homology: mouse and human.
- Clone:
- 11C253 (K28/43)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. PSD95 (postsynaptic density 95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels. Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5ug/ml detects PSD95 on 10ug of rat brain tissue lysate. Rat brain lysate was probed with N5380-25. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunohistochemistry: 1:500 detects PSD95 in rat cortex tissue. Optimal dilutions to be determined by the researcher. Positive Control: Rat brain tissue lysate Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice