P4700-08F-FITC

pan-MAGUK (Postsynaptic Density Protein 95, PSD-95, PSD95, Discs Large Homolog 4, DLG4, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90) (FITC)

Size

£1,070.00

SKU:: P4700-08F-FITC
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins.
Clone:: K28/86
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml detects members of the MAGUK protein family on 10ug of rat brain tissue lysate. Immunohistochemistry (paraffin): 1:500 detects the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues. Optimal dilutions to be determined by the researcher. Positive Control: Rat brain tissue lysate Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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