P4453-02-ML490
Polypyrimidine Tract Binding Protein (PTB) (MaxLight 490)
Size
£971.00
- SKU:
- P4453-02-ML490
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human PTB protein.
- Clone:
- 3G254
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The perinucleolar compartment (PNC) is a structure localized at the periphery of the nucleolus. Thus far the PTB (polypyrimidine tract binding protein) as well as small RNAs (i.e, Y RNA’s, MRP RNA, and Rnase P H1 RNA) have been identified in PNC. Studies conducted to characterize the structure of PNC revealed that RNA binding may be required for PTB to be localized in the PNC. Applications: Suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunoprecipitation: Native only Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice