P4350-25

PNPO, Recombinant, Human (Pyridoxine-5'-phosphate Oxidase, Pyridoxamine-phosphate Oxidase)

Size

£607.00

SKU:: P4350-25
Molecular Weight:: 25.9
Purity:: ~90% (SDS-PAGE).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 0.1M sodium chloride, 0.1mM PMSF.
Extra Details:: PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Source: Recombinant corresponding to aa57-261 of human PNPO protein, fused to His-tag at N-terminus, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MDPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP Molecular Weight: 25.9kD (226aa) confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher) Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.