P4350-15F

Pigment Epithelium-derived factor (PEDF, Serpin-F1, EPC-1)

Size

£607.00

SKU:: P4350-15F
Purity:: Purified by Immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to 413aa with an N-Terminal His-Tag from human Pigment Epithelium-derived factor, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: PEDF is syntetized and released by human fetal retinal pigment epithelial cells (RPE) into the interphotoreceptor matrix and is localized to human chromosome 17p. It is a 50kD multifunctional glycoprotein belonging to the serpin protease inhibitor supergene (serpin) family, acting like substrates rather than inhibitors of serine proteases, being also described as serine peptidase inhibitor, clade F (alfa-2 antiplasmin, pigment epithelium derived factor), member 1. Applications: Suitable for use in Indirect ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MRGSHHHHHH GMASQNPASP PEEGSPDPDS TGALVEEEDP FFKVPVNKLA AAVSNFGYDL YRVRSSMSPT TNVLLSPLSV ATALSALSLG AEQRTESIIH RALYYDLISS PDIHGTYKEL LDTVTARQKN LKSASRIVFE KKLRIKSSFV APLEKSYGTR PRVLTGNPRL DLQEINNWVQ AQMKGKLARS TKEIPDEISI LLLGVAHFKG QWVTKFDSRK TSLEDFYLDE ERTVRVPMMS DPKAVLRYGL DSDLSCKIAQ LPLTGSMSII FFLPLKVTQN LTLIEESLTS EFIHDIDREL KTVQAVLTVP KLKLSYEGEV TKSLQEMKLQ SLFDSPDFSK ITGKPIKLTQ VEHRAGFEWN EDGAGTTPSP GLQPAHLTFP LDYHLNQPFI FVLRDTDTGA LLFIGKILDP RGP Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice