P4350-15

PNPLA2 (PEDFR, Patatin-like phospholipase domain containing 2, 1110001C14Rik, Adipose triglyceride lipase, ATGL, Calcium-independent phospholipase A2, desnutrin, Desnutrin, DESNUTRIN, DKFZp667M109, FP17548, IPLA2-zeta, Patatin-like phospholipase domain-containing protein 2 Pigment epithelium-derived factor, Transport-secretion protein 2, TTS2, TTS2.2, TTS-2.2)

Size

£856.00

SKU:: P4350-15
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant corresponding to aa162-253 from mouse PNPLA2, expressed in E. coli (Q8BJ56).
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin) is a 55kD member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486aa type II transmembrane (TM) protein. Its TM segment is stated to span aa9-29. Alternatively, it may have a hydrophobic lipid-association domain between aa 267-295. It contains a palatin domain (aa10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of aa163-196 and 253-308, respectively. Over aa 62-253, mouse PEDF R shares 98% and 93% aa identity with rat and human PEDF R, respectively. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml Immunohistochemistry: 5-15ug/ml detects PNPLA2 in cells and tissues Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant mouse and recombinant human PNPLA2 is ~1ng/well Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice