P4296-06B

PML (Promyelocytic Leukemia, MYL, PP8675, Probable transcription factor PML, RING finger protein 71, RNF71, TRIM19, Tripartite motif-containing protein 19, Tripartite motif protein )

Size

£678.00

SKU:: P4296-06B
Application:: WB
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant partial human PML.
Clone:: B3E9
Format:: Supplied as a lyophilized powder from 0.01M PBS, pH 7.4. Reconstitute with 500ul of sterile 40-50% glycerol, ddH2O.
Extra Details:: PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The t(15;17)(q22;q11-q12) chromosomal trans-location of the retinoic acid receptor alpha gene occurs in virtually all cases of acute promyelocytic leukemia. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000 to differentiate normal PML and chimeric PML/RARa molecules based on their molecular weight. Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.