P4278-02
PLTP (Plasma Phospholipid Transfer Protein)
Size
£843.00
- SKU:
- P4278-02
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- NS0-derived Recombinant protein corresponding to aa18-493 with a Glu18Val substitution from human PLTP expressed in NS0 cells. Species Sequence Homology: porcine; 92%, mouse; 83%
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- PLTP (phospholipid transfer protein) is a 65-81kD, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature human PLTP is 476aa in length. It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain. There is one essential intrachain disulfide bond (Cys146-Cys185). There are four potential PLTP splice variants. Two show alternate start sites at Met21 and Met89. Two others demonstrate aa substitutions; one shows a one aa (Lys) substitution for aa110-162, and a second shows a four aa substitution for the N-terminal 35aa. Over aa18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice