P4275-07C
Plk3 (CNK, FNK, PRK, Polo-like Kinase 3)
Size
£837.00
- SKU:
- P4275-07C
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant protein corresponding to Pro320-Ala520 from human PLK-3.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Polo-like kinases are critical regulators of cell cycle progression, cytokinesis, mitosis, and the genotoxic stress response. Four distinct members have been identified to date in mammalian cells: PLK-1, PLK-2, PLK-3, and PLK-4. PLK-3 (polo-like kinase 3) plays an important role in cell cycle regulation by modulating microtubule dynamics and centrosomal function. Although protein levels are unchanged throughout the cell cycle, kinase activity peaks during late S and G2 phases. Many key cell cycle regulators are phosphorylated by PLK-3 including p53 on Ser20, Cdc25C on Ser216, and the major subunit of DNA polymerase delta, p125 on Ser60. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice