P4275-05
Plk (Polo-like Kinase) mixed clones
Size
£800.00
- SKU:
- P4275-05
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- A His-tagged Plk fusion protein (C-term Plk1) corresponding to aa402-603 of the human Plk1 protein.
- Clone:
- PL6/PL2 (3G251)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Plk is a serine/threonine kinase which is highly homologous to the Drosophila serine/threonine kinase encoded by the mutant polo. Plk and polo encode proteins of similar size and possess a similar overall structure, with their catalytic domains located near the N-terminal region. In Drosophila, the polo kinase appears to be involved in cell cycle regulation and chromosome segregation, and homozygous mutations in polo results in the appearance of aberrant chromosome segregation and frequently lethal mitosis. Studies on mammalian cells indicate that Plk expression is restricted to proliferating cells. Consistent with this finding, Plk mRNA can be detected in most tissues during mammalian development; however, in adult tissues, its expression is restricted to the thymus and ovaries. Plk is related to several other serine/threonine kinases which include: Saccharomyces cerevisiae CDC5, murine Snk (serum-inducible kinase), murine Fnk (FGF-inducible kinase), and murine Sak (Snk/Plk-akin kinase. Plk protein levels are cell-cycle regulated with low levels of detected in G1 phase and higher levels detected in S and G2-M. Plk kinase activity is also cell-cycle regulated and is modulated, at least in part, by phosphorylation of Plk on one or more serine residues by an as yet unidentified kinase. Phosphorylation on serine serves to stimulate Plk kinase activity and results in slower migration of the Plk protein when analyzed by SDS-PAGE. Interestingly, activation of the Plk kinase activity parallels that of Cdc2; however, Cdc2 kinase is not thought to be directly responsible for Plk phosphorylation. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1-5ug/ml Immunocytochemistry: 1-5ug/ml Immunohistochemistry (Paraffin): 1:10-1:100 Flow Cytometry: 3-5ug/ 10e6 cells ELISA: 0.1-1ug/ml Western Blot: 1-2ug/ml Immunoprecipitation: 5ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice