P4258-26

Platelet Derived Growth Factor Receptor alpha (PDGFRa)

Size

£767.00

SKU:: P4258-26
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa1035-1053, C-GKRNRHSSQTSEESAIETG of human Platelet Derived Growth Factor Receptor alpha, conjugated to KLH. Species Sequence Homology: frog, chicken, rat and zebra fish, which is different from the human sequence by one amino acid.
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Two types of PDGF receptors have been identified: PDGF Receptor alpha (PDGFRa) and PDGF Receptor beta (PDGFRb). The PDGF Receptor alpha, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGF Receptor beta, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity. Both receptors are transmembranous, ligand activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains. The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500-1:1000 dilution detected PDGF Receptor Type A, a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4258-26 (1:500). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 5-10ul immunoprecipitates Platelet Derived Growth Factor Receptor alpha from 500ug of 3T3/NIH RIPA lysate. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice