P4258-19
Platelet Derived Growth Factor AA,AB,BB Neutralizing (PDGF-AA,AB,BB)
Size
£269.00
- SKU:
- P4258-19
- Purity:
- Purified by DEAE chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified human PDGF-AB.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.0.
- Extra Details:
- Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. Applications: Suitable for use in Western Blot and Growth Inhibition Assays. Other applications have not been tested. Recommended Dilutions: Western Blot: 5-10ug/ml, under non-reducing conditions, detects 100ng of human PDGF-AB (MW~28kD) as well as 100ng of human PDGF-BB (MW~30-32kD). Growth Inhibition Assay: In a 5 day assay, 10-20ug/ml inhibited 3T3 cell growth by 100% as stimulated by 10ng/ml of PDGF-AB. Opimal dilutions to be determined by the researcher. Growth Inhibition Assay Protocol: 1. In DMEM, 1% calf serum, preincubate PDGF-AB (10ng/ml) and P4258-19, PDGF (10-20ug/ml) for one hour at 37°C. Serially dilute P4258-19 2-fold from 40ug/ml to 2.5ug/ml in a 96 well flat black view plate. 2. Prepare a 3T3 cell suspension at 10e4 cells/ml and aliquot 100ul to each well. 3. Refeed on Day 3. 4. Count wells on day 5 using an ATP endpoint assay. Wash cell layer once with PBS prior to lysis. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice