P4258-19-ML650

Platelet Derived Growth Factor AA,AB,BB Neutralizing (PDGF-AA,AB,BB) (MaxLight 650)

Size

£1,075.00

SKU:: P4258-19-ML650
Purity:: Purified by DEAE chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified human PDGF-AB.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis. Applications: Suitable for use in Western Blot and Growth Inhibition Assays. Other applications have not been tested. Recommended Dilution: Western Blot: under non-reducing conditions, detects 100ng of human PDGF-AB (MW~28kD) as well as 100ng of human PDGF-BB (MW~30-32kD). Growth Inhibition Assay: In a 5 day assay, 10-20ug/ml inhibited 3T3 cell growth by 100% as stimulated by 10ng/ml of PDGF-AB. Opimal dilutions to be determined by the researcher. Growth Inhibition Assay Protocol: 1. In DMEM, 1% calf serum, preincubate PDGF-AB (10ng/ml) and P4258-19, PDGF (10-20ug/ml) for one hour at 37°C. Serially dilute P4258-19 2-fold from 40ug/ml to 2.5ug/ml in a 96 well flat black view plate. 2. Prepare a 3T3 cell suspension at 10e4 cells/ml and aliquot 100ul to each well. 3. Refeed on Day 3. 4. Count wells on Day 5 using an ATP endpoint assay. Wash cell layer once with PBS prior to lysis. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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