P4258-15F

Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

Size

£594.00

SKU:: P4258-15F
Molecular Weight:: 24.3
Purity:: ≥98% (SDS-PAGE and HPLC). Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder.
Extra Details:: Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant protein corresponding to human PDGF-BB, a disulfide-linked homodimer of two B chains, 218 total amino acids, expressed in E. coli. Molecular Weight: ~24.3kD Biological Activity: The ED50 as determined by the dose-dependent stimulation of BALB/c 3T3 cells proliferation is < 1ng/ml Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.