P4258-03F

Platelet Derived Growth Factor Receptor, alpha (PDGFRa) (Carboxyfluorescein)

Size

£709.00

SKU:: P4258-03F
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human osteosarcoma cell membrane extracts
Clone:: 7H37
Format:: Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein.
Extra Details:: PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF Rα binds all three PDGF isoforms with high affinity, PDGF Rβ binds PDGF-BB and AB, but not PDGF-AA. Both PDGF Rα and PDGF Rβ are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Only PDGF Rα is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-Rα has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF Rα binds PDGF with high affinity and is a potent PDGF antagonist. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.

Shipping Conditions:: Blue Ice