Plasminogen (Pg)

Size

£544.00

SKU:: P4256-45
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Human Plasminogen purified from plasma.
Format:: Supplied as a liquid in 0.01M HEPES, pH 7.4, 0.15M sodium chloride and 50% glycerol.
Extra Details:: Plasminogen (Pg) is synthesized in the liver and circulates in plasma at a concentration of ~200ug/ml (~2.3uM). Plasminogen is a single-chain glycoprotein of ~88kD that consists of a catalytic domain followed by five kringle structures. Within these kringle structures are four low-affinity lysine binding sites and one high-affinity lysine binding site. It is through these lysine binding sites that plasminogen binds to fibrin and to alpha-2-antiplasmin. Native plasminogen (glu-plasminogen) exists in two variants that differ in their extent of glycosylation, and each variant has up to six isoelectric forms with respect to sialic acid content, for a total of 12 molecular forms. Activation of glu-plasminogen by the plasminogen activators urokinase (UPA), or tissue plasminogen activator (tPA) occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. In a positive feedback reaction, the plasmin generated cleaves an ~8kD peptide from glu-plasminogen, producing lys77-plasminogen which has a higher affinity for fibrin and when bound is a preferred substrate for plasminogen activators such as urokinase. Additional activators of plasminogen include kallikrein and activated factor XII. The primary inhibitor of plasmin in plasma is alpha-2-antiplasmin. Other physiological inhibitors of plasmin include alpha-2-macroglobulin and antithrombin1-3. Applications: Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice