Plasminogen Activator Inhibitor Type 1 (PAI 1) (HRP)

Size

£588.00

SKU:: P4256-31A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant Plasminogen Activator Inhibitor Type 1 (PAI 1) from bacterial extracts.
Format:: Supplied as a liquid in buffered stabilizer solution, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Extra Details:: Type I plasminogen activator inhibitor, PAI 1, is a 50kD single- chain glycoprotein which belongs to the serine protease inhibitor (SERPIN) family of proteins. The concentration of PAI 1 in normal human plasma is very low, with an average concentration of ~20ng/ml. The plasma concentrations of PAI 1 can be affected by a number of factors including diurnal variations, age, sex, pregnancy, obesity and exercise status. PAI 1 is synthesized by various cell types including endothelial cells, hepatocytes, vascular smooth muscle cells, fibroblasts, mesothelial cells, granulosa cells and malignant cell lines. It is also found in the alpha-granules of platelets as well as plasma. PAI 1 exists in at least three different conformations, including an active form with a half-life of ~1-3 hours, a latent form and a proteolytically or oxidatively inactivated form. The plasma glycoprotein vitronectin has been shown to bind active PAI 1 and stabilize it in its active conformation, extending its functional half-life to greater than 24 hours. Little specific binding of the latent or inactive forms of PAI 1 to vitronectin occurs. PAI 1 is considered to be the primary regulator of plasminogen activation in vivo. It is the major physiologic inhibitor of both the single chain and two-chain forms of tPA, being able to inhibit the single-chain form at least 10000 times greater than other PAIs. Applications: Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Rz Ratio (Reinheitszahl, A403/A280): 0.41 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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