Plasminogen Activator Inhibitor Type 1 mutant, vitronectin binding (PAI-1)

Size

£1,558.00

SKU:: P4256-29N
Molecular Weight:: 43
Storage Conditions:: -70°C
Supplier:: United States Biological
Extra Details:: Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6). Physical Appearance: Colorless frozen solution Solubility: >2mg/ml and <5mg/ml Buffer Composition: 0.05M sodium phosphate; 0.1M sodium chloride; 1mM EDTA; pH 6.6 Purity: >98% pure by SDS PAGE 95 (+/-) 5% active by uPA titration. Absorbance (280nm): 3.6 E0.1% = 1.0 Concentration = 3.6mg/ml Active Concentration = 3.4mg/ml Kinetic Data: Second order rate constants for inhibition of: UPA = 6.3 X 106 M^-1s^-1 tPA = 5.7 X 106 M^-1s^-1 Storage and Stability: Store frozen at -70°C – stability >1 year.