P4230-14-ML650
Plakophilin-3 (MaxLight 650)
Size
£971.00
- SKU:
- P4230-14-ML650
- Purity:
- Purified by Protein A affinity chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the Armadillo domain of human Plakophilin-3.
- Clone:
- 6A161
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Plakophilin-3 is a novel 87kD Armadillo-like (Arm) protein, which is characterized by a series of repeats of 42-45 a.a., originally described in the Drosophila protein Armadillo. Plakophilin-3 is a member of the p120ctn/plakophilin subfamily, which includes plakophilin-1, plakophilin-2, the ARVCF gene product and d-catenin. This protein is similar to plakophilin-1 and plakophilin-2. Other Arm proteins include b-catenin and a-catenin. All these proteins are part of the cell-cell adhesion complex . On Western Blot analysis, plakophilin-3 was expressed in epithelial cell lines but not in foreskin fibroblasts and sarcomaderived cell lines. By Immunofluorescence, plakophilin-3 was colocalized with desmoglein in desmosomes of epithelial cells Applications: Suitable for use in FLISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilution: FLISA: 0.1-1ug/ml Immunoprecipitation: 10ug/IP reaction Immunohistochemistry: 10ug/ml Immunoflluorescence: 1-3ug/ml Western Blot: 1-3ug/ml Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2% Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice