P4230-14-BIOTIN
Plakophilin-3 (Biotin)
Size
£971.00
- SKU:
- P4230-14-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the Armadillo domain of human Plakophilin-3.
- Clone:
- 6A161
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Extra Details:
- Plakophilin-3 is a novel 87kD Armadillo-like (Arm) protein, which is characterized by a series of repeats of 42-45 a.a., originally described in the Drosophila protein Armadillo. Plakophilin-3 is a member of the p120ctn/plakophilin subfamily, which includes plakophilin-1, plakophilin-2, the ARVCF gene product and d-catenin. This protein is similar to plakophilin-1 and plakophilin-2. Other Arm proteins include b-catenin and a-catenin. All these proteins are part of the cell-cell adhesion complex . On Western Blot analysis, plakophilin-3 was expressed in epithelial cell lines but not in foreskin fibroblasts and sarcomaderived cell lines. By Immunofluorescence, plakophilin-3 was colocalized with desmoglein in desmosomes of epithelial cells Applications: Suitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: 0.1-1ug/ml Immunoprecipitation: 10ug/IP reaction Immunohistochemistry: 10ug/ml Immunoflluorescence: 1-3ug/ml Western Blot: 1-3ug/ml Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2% Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice