P4230-13

Plakophilin 3

Size

£659.00

SKU:: P4230-13
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa570-589, CFTPQSRRLRELPLAADALTF of human plakophilin 3 (KLH via an additional cysteine at the N-terminus).
Clone:: 4A82
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Extra Details:: Several major families of cell adhesion molecules can be distinguished. These include the cadherins and N-CAMs, which play a crucial role in cell recognition and adhesion, while integrins are implicated in the adherence of cells to the extracellular matrix. Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. NCAM’s (neural cell adhesion molecules) exist in at least three forms of plasma membrane plycoproteins, which are expressed in a variety of tissues including most nerve cells. The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an a- and a beta-subunit in interconnecting the cytoskeleton and the extracellular matrix. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry: Methanol and paraformaldehyde-fixed cells. Immunohistochemistry (Frozen and paraffn embedded): 1:25-1:200 (ABC) Western Blot: 1:100-1:1000 Note: Pretreatment with 0.2% Triton-X 100 for 5 minutes for fixed cells and 15 minutes for frozen tissues, prior to incubation with primary antibody. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice