Plectin (Not for Export EU)

Size

£877.00

SKU:: P4214-31
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Guinea Pig
Immunogen:: C-terminal “C” domain of human recombinant plectin (aa4367-4684).
Format:: Supplied as a liquid, 0.5% BSA, 0.09% sodium azide.
Extra Details:: Plectin, a 500kD intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4). Plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes). Applications: Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:10,000 Immunohistochemistry (frozen and paraffin sections, enhanced after microwave treatment): 1:500: Incubate for 1 hr at RT; extended with paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice